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Long-Term Open-Top-Chamber Study of Sour Orange Trees.

In July of 1987, as described by U.S. Department of Agriculture researchers Idso and Kimball (2001) , eight 30-cm-tall sour orange tree (Citrus aurantium L.) seedlings were planted directly into the ground at the Agricultural Research Service's U.S. Water Conservation Laboratory in Phoenix, Arizona, where they were enclosed in pairs within four clear-plastic-wall open-top chambers. Then, in November of that year, the two scientists began to continuously pump ambient air through two of the chambers via perforated plastic tubes that lay upon the ground beneath the trees, while through the other two chambers they began to pump air that was enriched with carbon dioxide to a concentration that was 300 ppm greater than that of the surrounding ambient air, which had an average CO2 concentration of 400 ppm. And thus was born one of the longest atmospheric CO2 experiments ever to be conducted anywhere in the world.

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